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Image Search Results
Journal: Cell Death & Disease
Article Title: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression
doi: 10.1038/cddis.2014.370
Figure Lengend Snippet: MEIS2 is essential for neuroblastoma cell survival. ( a ) Immunoblotting of MEIS2 in a panel of 13 human neuroblastoma cell lines. β -Actin levels are shown as a loading control. ( b and c ) qRT-PCR ( b ) and immunoblot ( c ) analyses of MEIS2 expression in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. Error bars ( b ) represent S.D. ( n =3). GAPDH ( c ) serves as a loading control. ( d ) Micrographs of BE(2)-C cells infected for 3 days with lentiviruses expressing either shGFP or shMEIS2. ( e ) Trypan blue exclusion assay of viable BE(2)-C cells infected for 4 days with lentiviruses expressing either shGFP or shMEIS2
Article Snippet: Immunoblotting was conducted according to standard procedures using the following primary antibodies: rabbit anti-BCL2 (sc-783, 1 : 200), rabbit anti-caspase 3 (sc-7148, 1 : 200), rabbit anti-Flag (F7425, 1 : 1000; Sigma-Aldrich), rabbit anti-FOXM1 (sc-502, 1 : 100), rabbit anti-GAPDH (sc-25778, 1 : 3000), mouse anti-MEIS2 (sc-81986, 1 : 400),
Techniques: Western Blot, Quantitative RT-PCR, Expressing, Infection, Trypan Blue Exclusion Assay
Journal: Cell Death & Disease
Article Title: MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression
doi: 10.1038/cddis.2014.370
Figure Lengend Snippet: MEIS2 targets the MuvB-BMYB-FOXM1 complex for transcriptional control of M-phase progression. ( a ) qRT-PCR analysis of mRNA expression of MEIS2, FOXM1, BMYB, and RBBP4 in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. ( b ) Immunoblotting of MEIS2 and FOXM1 in BE(2)-C cells infected with lentiviruses expressing either shGFP or shMEIS2. β -Actin levels are shown as a loading control. MEIS2 and FOXM1 levels were quantified against β -actin. ( c ) GSEA showing marked downregulation of the FOXM1 pathway genes in BE(2)-C cells with MEIS2 depletion. ( d ) ChIP-qPCR analysis showing MEIS2 binding to the FOXM1 promoter region (−312 to −158) containing a consensus TGIF/MEIS2-binding sequence TGTCA. Error bars, S.D. ( n =3). ( e ) Immunoblotting of FOXM1 in BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. β -Actin levels are shown as a loading control. ( f ) qRT-PCR analysis of mRNA expression of key FOXM1 target genes in BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. ( g ) Growth assay of BE(2)-C and SK-N-DZ cells with or without FOXM1 knockdown. Error bars, S.D. ( n =3). ** P <0.001. Data ( d and g ) were analyzed with two-tailed Student's t -test
Article Snippet: Immunoblotting was conducted according to standard procedures using the following primary antibodies: rabbit anti-BCL2 (sc-783, 1 : 200), rabbit anti-caspase 3 (sc-7148, 1 : 200), rabbit anti-Flag (F7425, 1 : 1000; Sigma-Aldrich), rabbit anti-FOXM1 (sc-502, 1 : 100), rabbit anti-GAPDH (sc-25778, 1 : 3000), mouse anti-MEIS2 (sc-81986, 1 : 400),
Techniques: Quantitative RT-PCR, Expressing, Infection, Western Blot, Binding Assay, Sequencing, Growth Assay, Two Tailed Test